Login to your account

Username
Password
Remember Me

Create an account

Fields marked with an asterisk (*) are required.
Name
Username
Password
Verify password
Email
Verify email

Warning: Creating default object from empty value in /home4/sameerp/osites/ivfodisha.com/components/com_k2/models/item.php on line 494

ICSI (intracytoplasmic sperm injection)

Vote it
(0 votes)
Written by  Published in: Category 1
icsiThe most recent microassisted fertilization method is called intracytoplasmic sperm injection (ICSI) and involves the injection of a single sperm into the cytoplasm of the oocytes. ICSI has forever changed the management of male factor infertility. ICSI is now widely available in a large number of assisted conception units internationally and has revolutionized the management of male factor infertility. ICSI is associated with fertilization and pregnancy rates similar to those found following conventional IVF in patients who do not have male factor problems. In ICSI all the steps are similar to the procedure of IVF, except the step of fertilization. Normally in IVF one egg is mixed with 100,000 sperms and one of the sperms fertilizes the egg on its own. In contrast, in ICSI each egg is held and injected with a single live sperm. This micro-fertilization is done with the help of a machine called the Micromanipulator.

IVF Odisha provides comprehensive affordable ICSI treatment and services. ICSI Centre in India, Our clinic deals in Infertility treatment which includes ICSI treatment

Thus the procedure consists of:
1. Controlled Ovarian stimulation with drugs (GnRH Analogues and Gonadotrophins) to produce many eggs.
2. Monitoring of follicles and egg development with the aid of vaginal sonography and serial Estradiol hormone estimation.
3. Administration of hCG injection, (Human Chorionic Gonadotrophins) when the two leading follicles are 18mm in diameter.
4. Oocyte or egg retrieval under short general anesthesia 35 to 37 hours after HCG injection.
5. Identification and isolation of eggs in the laboratory.
6. Sperm collection and processing in the lab. Incase of azoospermia (no sperms in the semen) the sperms are collected directly from the testis with the procedures of PESA/MESA/TESE or TESA.
7. Dissection of the eggs in the laboratory with the help of an enzyme called Hyloronetis. Placement of eggs into small droplets of culture media under oil.
8. Placement of sperms into small droplets of PVP under oil. Immobilization of the sperm with a micro-injection needle (Diameter of 7 microns) and aspiration of the immobile sperm into the needle (tail first).
9. Holding the egg with a holding pipette and injection of the immobilized sperm into the held egg Placement of these eggs into the incubator for 2 to 5 days.
10. Embryo formation 2 to 5 days after fertilization.
11. Embryo transfer of good quality embryos back to the womb, after 2 (four cell embryo), 3 (six-eight cell embryo)or 5(blastocyst stage) days after egg removal.

Indications:
1. Males with severe sperm factors such as low count (less than 5 million), very poor motility or high degree of abnormal sperms.
2. Males with azozoospermia, where there is no sperm present in the semen. The azozoospermia may be of the obstructive type where there is production of sperms in the testis but blockage of the conduction system which brings the sperm out into the semen. Alternately, the azoospermia may be of the non-obstructive type, where there is a failure of the testis to produce sperms. Nowadays, in both these types of azoospermia, sperms can be isolated directly from the testis, using the SPERM

Retrieval Techniques of PESA/TESA/TESE and subsequently, ICSI can be performed on:
1. Males with sperm anti-bodies.
2. Males with ejaculated dysfunction due to spinal chord injury or malfunction such as quadriplegics or paraplegics.
3. Patients with retrograde ejaculation (ejaculation of the sperm into the urinary bladder) who fail to become pregnant with IUI.
4. Patients where fertilization has failed with In Vitro Fertilization.

In our unit we also believe in doing ICSI on patients who have had previous history of tuberculosis or endometriosis as we believe it gives better fertilization rates than standard IVF (this is a personal experience not supported by international literature). Now a days, some units are advocating routine ICSI for all patients, including those with normal sperm counts. We do not believe in such practice as we feel that pregnancy should be achieved with minimum handling of the gametes outside the body. If the sperm count is good enough for fertilization with IVF, we will not do ICSI. However, if a particular patient has a sperm count which is in the grey-zone area, then we may subject half the eggs to IVF and half the eggs to ICSI. Our success rates are in the region of 30 to 40% in both azoospermia and non-azoospermia patients.

Concept:
Similar to IVF, ICSI differs in the fertilization process. Unlike in IVF, where one egg is mixed with 1 lakh sperms, with fertilization taking place on its own, ICSI is a technique where each egg is held and injected with a single live sperm. This micro-fertilization is done with the help of a machine called the Micromanipulator. The procedure (anchor) can be categorised into 11 steps.

Indications for ICSI
ICSI is a technique usually performed in males with:

severely low sperm counts
poor quality of sperms
more… link as an anchor to the retrieval techniques of PESA / MESA etc, lower down in the content paras.

ICSI and IVF Odisha

In ICSI all the steps are similar to the procedure of IVF (procedure of IVF), except in fertilization.

Procedure:
Controlled Ovarian stimulation with drugs (GnRH Analogues and Gonadotrophins) to produce many eggs.
Monitoring of follicles and egg development with the aid of vaginal sonography and serial estradiol hormone estimation.
Administration of hCG injection, (Human Chorionic Gonadotrophins) when the two leading follicles are 18mm. in diameter.
Oocyte or egg retrieval under short general anaesthesia, 35 to 37 hours after HCG injection.
Identification and isolation of eggs in the laboratory.
Sperm collection and processing in the lab. Incase of azoospermia (no sperms in the semen) the sperms are collected directly from the testis with the procedures of PESA/MESA/FTNB/TESE or TESA.
Dissection of the eggs in the laboratory with the help of an enzyme called Hyloronetis Placement of eggs into small droplets of culture media under oil.
Placement of sperms into small droplets of PVP under oil. Immobilisation of the sperm with a micro-injection needle (Diameter of 7 microns) and aspiration of the immobile sperm into the needle (tail first).
Holding the egg with a holding pipette and injection of the immobilized sperm into the held egg Placement of these eggs into the incubator for 2 to 5 days.
Embryo formation 2 to 5 days after fertilization.
Embryo transfer of good quality embryos back to the womb, after 2(four cell embryo), 3 (six-eight cell embryo)or 5(blastocyst stage) days after egg removal.

Indications:
1. Males with severe sperm factors such as:
low count (less than 5 million)
very poor motility
high degree of abnormal sperms.

Although ISCI is carried out among patients even with normal sperm counts, IVF Odisha believes that pregnancy should be achieved with a minimum handling of the gametes outside the body. If a particular patient has a sperm count that is in the grey-zone area, then we may subject half the eggs to IVF and half the eggs to ICSI.

Males with azoospermia have no sperm present in the semen. The azoospermia may be of the obstructive type where there is production of sperms in the testis but a blockage in the conduction system disallows sperms to enter the semen. Alternately, the azoospermia may be of the non-obstructive type, where there is a failure of the testis to produce sperms. Fortunately, today, sperms can be isolated directly from the testis, using the Sperm Retrieval Techniques of PESA/TESA/TESE and subsequently, ICSI can be performed. IVF Odisha maintains a competent success rate of 30-40% in males with azoospermia.

2. Males with sperm anti-bodies.

3. Males with ejaculated dysfunction due to an injury to the spinal chord or in quadriplegics or paraplegics.

4. Patients with retrograde ejaculation (ejaculation of the sperm into the urinary bladder) who fail to allow pregnancy under.

5. Patients where In Vitro Fertilisation has proved to be unsuccessful.

6.  At IVF Odisha, ICSI is performed for on patients with a history of tuberculosis or endometriosis as we believe ICSI shows higher fertilization rates than standard IVF.
Read 126683 times Last modified on Wednesday, 30 May 2012 19:21

19709 comments

  • Comment Link Friday, 08 December 2017 05:44

    I have been exxploring for a Ƅit for ɑny hiցh-quality articles օr blog posts οn tһis sort off
    area . Explorinng in Yahoo I fіnally stumbled uроn this web site.
    Studying tһis infօrmation So i am һappy to snow that I haᴠe
    a very excelkent uncanny feeling I cane
    upon just ѡhat I needеd. I moѕt indisputably
    wіll ake certain to ԁon?t putt oout ⲟf youг mind this web site and pгovides іt a looҝ regularly.

  • Comment Link Friday, 08 December 2017 03:48

    I ordered this looking for a great gifts for my mama. Ones headphone came within a perfect purple tied up container. This was a searching gifts under the actual christmas tree!! That the headphone also looked stunning, however my mom was gigantic boned as well as the headphone is a little tight, still it is a great present! I appreciate this.

  • Comment Link Friday, 08 December 2017 03:44

    Please keep on posting such quality stories as this is
    a rare thing to find today. I am always searching online for blogposts that
    may assist me. Looking forward to another good website.
    All the best!

  • Comment Link Friday, 08 December 2017 03:16

    I paid for this looking for a awesome gift for the the mother. Some sort of headphone arrived in a gorgeous purple tied box. This is the best hunting gift under that christmas time tree!! That headphone always looked striking, however my mother was gigantic boned as well as the headphone was somewhat tight, then again the outstanding present! I really like that it.

  • Comment Link Friday, 08 December 2017 03:13

    Great post, you have stated some excellent points ,
    I additionally think this a very superb website.

  • Comment Link Friday, 08 December 2017 02:54

    You couⅼɗ dеfinitely ѕee you skills wthin the article ʏou ԝrite.
    Thе wⲟrld hopes fօr evеn mߋre passionate writrs lіke
    you wһo aren't afraid to say hⲟw theʏ beⅼieve.

    All thhe time go after yoսr heart.

  • Comment Link Friday, 08 December 2017 02:43

    Oh my goodness! a great article man. Thank you for sharing it.

  • Comment Link Friday, 08 December 2017 01:32

    Today, while I was at work, my cousin stole my iPad and tested to see if
    it can survive a twenty five foot drop, just so she can be a
    youtube sensation. My iPad is now broken and she has 83 views.

    I know this is completely off topic but I had to share it with someone!

  • Comment Link Friday, 08 December 2017 01:13

    obviously lіke your web site however yyou need to take ɑ lоoқ at
    tһe spelling oon գuite a few of your posts. Several ߋf them are rife
    ᴡith spelling prⲟblems and I tоο find іt very troublesome tⲟ infor
    tһe truth on thе other hɑnd I'll certainlү come bаck aɡain.

  • Comment Link Thursday, 07 December 2017 23:55

    I don't leave loads of comments on a lot of blogs however
    i felt i had to here. Quality articles you have here.

Leave a comment

Make sure you enter the (*) required information where indicated. HTML code is not allowed.




Team IVF Odisha

drpltripathy
Dr. P. L. Tripathy
Obstetrician & Infertility Specialist
  • Dr. Neeraj Pahlajani
    Obstetrician & Infertility Specialist

    dr sameerpahlajaniDr. Sameer PahlajaniInfertility Consultant & Sonography ExpertDr Dibyajit_MohantyDr. Dibyajit MohantyMD, Obs. & GynecDr Sasmita_DasDr. Sasmita DasMD, Obs. & Gynec